Composite

Part:BBa_K5166032:Design

Designed by: Sihui Liu; Yancheng Zeng; Xiao Han; Zhirong Wang   Group: iGEM24_BIT-China   (2024-09-29)


Pichia Pastoris GS115 Surface Display System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 751
    Illegal NotI site found at 1240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1360
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 238


Design Notes

We constructed a surface display system that could link target proteins to anchor proteins to be displayed on the surface of Pichia pastoris.

Source

We designed the surface display system.

References

[1]Hossain, S. A., Rahman, S. R., Ahmed, T., & Mandal, C. (2020). An overview of yeast cell wall proteins and their contribution in yeast display system. Asian Journal of Medical and Biological Research, 5(4), 246-257. doi: 10.3329/ajmbr.v5i4.45261
[2] Andreu, C., & del Olmo, M. (2018). Yeast arming systems: pros and cons of different protein anchors and other elements required for display. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 102(6), 25Tabañag, I., Chu, I., Wei, Y., & Tsai, S. (2018). The Role of Yeast-Surface-Display Techniques in Creating Biocatalysts for Consolidated BioProcessing. CATALYSTS, 8(3). doi: 10.3390/catal8030094
[4] Kuroda, K., Matsui, K., Higuchi, S., Kotaka, A., Sahara, H., Hata, Y.,... Ueda, M. (2009). Enhancement of display efficiency in yeast display system by vector engineering and gene disruption. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(4), 713-719. doi: 10.1007/s00253-008-1808-4
[5] Tanaka, T., Matsumoto, S., Yamada, M., Yamada, R., Matsuda, F.,... Kondo, A. (2013). Display of active beta-glucosidase on the surface of Schizosaccharomyces pombe cells using novel anchor proteins. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(10), 4343-4352. doi: 10.1007/s00253-013-4733-0